taq polymerase การใช้

• PCR uses replication enzymes that are tolerant of high temperatures, such as the thermostable Taq polymerase.
• The suggestion that Mullis was solely responsible for the idea of using Taq polymerase in the Roche Molecular Systems.
• If Taq polymerase is good to go at 95 C, why does it need to be stored at-20?
• "Cold-sensitive Taq polymerase " : is a modified DNA polymerase with almost no activity at low temperature.
• Thermostable enzymes such as Taq polymerase and Pfu DNA polymerase are used in polymerase chain reactions where temperatures of 94 癈 or over are used to melt apart DNA strands.
• Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase ( an enzyme originally isolated from the bacterium " Thermus aquaticus " ).
• In the long term it is planned to enable Mongolian scientists to produce all substances needed for the continuation of the diagnosis themselves, such as recombinant diagnostic antigens, or Taq polymerase.
• The Klenow fragment was also the original enzyme used for greatly amplifying segments of DNA in the polymerase chain reaction ( PCR ) process, before being replaced by thermostable enzymes such as Taq polymerase.
• White pays tribute to Mullis's fertile mind, describing how he came up with practical ways to improve PCR, such as the use of Taq polymerase, an enzyme made by bacteria that live at high temperatures.
• My lab supervisor ( I'm an MSc student and she's a PhD student ) often seems obsessive about keeping Taq polymerase on ice when it's not in the freezer and minimising the time spent between.
• You all miss the key point : PCR machine manufacturers pay a license fee to the owners of the PCR and Taq polymerase patents . when you buy Taq, you only buy a license to use it in authorised PCR machines.
• The discovery in 1976 of Taq polymerase  a DNA polymerase purified from the thermophilic bacterium, " Thermus aquaticus ", which naturally lives in hot ( ) environments such as hot springs  paved the way for dramatic improvements of the PCR method.
• "Thermus aquaticus " is a bacterium found in the Yellowstone hot springs that produces an important enzyme ( Taq polymerase ) that is easily replicated in the lab and is useful in replicating DNA as part of the polymerase chain reaction ( PCR ) process.
• In 1986, Saiki started to use " Thermophilus aquaticus " ( Taq ) DNA polymerase to amplify segments of DNA . The Taq polymerase was heat resistant and would only need to be added once, thus making the technique dramatically more affordable and subject to automation.
• As an undergrad, I remember that one of my professors worked with a bacteria ( Archaea, actually ) sampled from hot springs in Italy, that grew best at 75癈 . And the infamous " T . aquaticus ", from which scientists get Taq polymerase, grows in the boiling hotsprings of Yellowstone National Park, and is perfectly happy for small stints at 95癈 . Tuckerekcut 17 : 23, 14 July 2006 ( UTC)